What are SEA LIFE Orlando opening and closing times? Standard Opening Hours: MONDAY TO SUNDAY, 10AM TO 9PM. LAST ENTRY IS AT 8PM. Open 7 days a week, 365 days a year. For our most up to date hours of operation please click HERE! Related articles What are the Coca-Cola Orlando Eye opening and closing times? Can I share my Merlin Annual Pass with someone else? I have recently applied for a job and would like to discuss my application, who can I contact?