What are SEA LIFE Orlando opening and closing times? Standard Opening Hours: MONDAY TO SUNDAY, 10AM TO 9PM. LAST ENTRY IS AT 8PM. Open 7 days a week, 365 days a year. For our most up to date hours of operation please click HERE! Related articles How long can I expect to wait in line to get into Madame Tussauds? Do you offer e-ticket / print at home option?